جامعة الكوت - University of kut - بحوث

السنة	عنوان البحث	نشر البحث
2026	INTEGRATED IN VITRO AND CLINICAL ISOLATE–BASED EVALUATION OF PROBIOTIC ANTIMICROBIAL ACTIVITY AGAINST SALMONELLA SPP. AND STAPHYLOCOCCUS SPP	AJPCR
Objective: To evaluate the antimicrobial activity of selected probiotic strains against clinical isolates of Salmonella spp. and Staphylococcus spp. and to assess their potential as complementary antimicrobial agents against clinically relevant bacterial pathogens. Methods: A total of 20 clinical isolates (10 Salmonella spp. and 10 Staphylococcus spp.) were evaluated against three probiotic strains: Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum. Antimicrobial activity was assessed using the agar well diffusion assay to measure inhibition zone diameters and the broth microdilution method to determine the minimum inhibitory concentration (MIC) expressed as percentage (% v/v) of cell-free supernatant (CFS). Statistical analysis was performed using one-way ANOVA followed by Tukey’s HSD test. Results: All tested probiotic strains demonstrated inhibitory activity against both Salmonella spp. and Staphylococcus spp. Lactobacillus acidophilus exhibited the strongest antimicrobial effect, producing the largest inhibition zones (20.3 ± 1.5 mm and 22.1 ± 1.2 mm, respectively) and the lowest MIC value (6.25% v/v). Lactobacillus casei showed moderate activity with inhibition zones of 16.2 ± 1.3 mm and 18.0 ± 1.4 mm and an MIC of 12.5% v/v, whereas Bifidobacterium bifidum exhibited comparatively lower activity with inhibition zones of 12.4 ± 1.1 mm and 14.2 ± 1.0 mm and an MIC of 25% v/v. Significant differences among probiotic strains were confirmed by ANOVA (p < 0.05). Conclusion: The findings indicate that probiotic strains possess significant antimicrobial activity against clinically relevant bacterial pathogens. Among t